The invention discloses a PVDF-based microneedle-type piezoelectric micro-force sensor and a preparation method thereof. The PVDF-based microneedle-type piezoelectric micro-force sensor comprises an upper electrode layer, a lower electrode layer, a glass microneedle tip and a piezoelectric layer. The upper electrode layer and the lower electrode layer are conductive layers respectively; the piezoelectric layer is made of polyvinylidene fluoride (PVDF) and binary polymer and multipolymer materials thereof; and the glass microneedle tip is a substrate of the micro-force sensor. The preparation method is characterized by carrying out glass capillary laser heating and drawing to form the glass microneedle tip; through sputtering, spin coating or evaporation process, preparing the lower electrode layer on the glass microneedle tip; preparing the PVDF and binary polymer and multipolymer material film layer through blade coating, spin coating or electrostatic spinning process; and preparing the upper electrode layer on the glass microneedle tip coated with the PVDF piezoelectric layer through sputtering, spin coating or evaporation process. The micro-force sensor has the advantages of simple structure, easy realization of processing technology, small size and high sensitivity and the like, and is especially suitable for detection of micro force caused by micro air flow and micro fluid.