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发明授权
CN106404962B 一种测定花生叶片茉莉酸含量的方法 有权

一种测定花生叶片茉莉酸含量的方法
摘要：
本发明公开了一种花生叶片中茉莉酸的检测方法，包括以下步骤：(1)花生叶片样品制备；(2)标准曲线样品制备；(3)制备流动相以及茉莉酸、二氢茉莉酸甲醇溶液；(4)样品的萃取与富集；(5)LC‑MS检测条件的建立；(6)样品检测。发明测试方法稳定性高，数据精确，检测结果可靠，化合物分离度较好。本发明方法能够快速检测花生叶中的茉莉酸含量，操作快速，效率高，具有重要的科学意义。
摘要（英）：

The invention discloses a detection method for jasmonic acid in peanut leaves. The method comprises the following steps of 1 preparation of a peanut leaf sample, 2 preparation of a standard curve sample, 3 preparation of a mobile phase, a jasmonic acid solution and a dihydrojasmonic acid methanol solution, 4 sample extraction and enrichment, 5 LC-MS detection condition construction and 6 sample detection. According to the detection method, the stability is high, data is accurate, a detection result is reliable, and the compound separation degree is good; the jasmonic acid content in the peanut leaves can be quickly detected, operation is rapid, the efficiency is high, and the important scientific significance is achieved.

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