The invention discloses a veterinary compound florfenicol injection, and belongs to the field of veterinary preparations. Every 1 L of the injection comprises 50 g to 300 g of florfenicol, 5 g to 30 g of flunixin meglumine, 300 ml to 600 ml of isopropyl myristate, 150 ml to 450 ml of polyethylene glycol stearate, 0.5 g to 3 g of anhydrous sodium sulfite and 50 ml to 200 ml of water for injection. A preparation method is disclosed. The veterinary compound florfenicol injection has an obvious effect on poultry and livestock bacteriosis, morbus virosus and parasitic disease concurrency prevention and treatment and secondary bacterium infection, and especially effectively treats pig infective pleuropneumonia necrotic enteritis, piglet's yellow-white dysentery, swine enzootic pneumonia, atrophic rhinitis, metritis-mastistis-agalactia, clostridial enteritis of piglets, postpartum infection, haemophilus parasuis and the like. The preparation process is simple, good stability is achieved, cost is low, the water for injection is used for replacing part of an organic solvent, local analgesic does not need to be added, good liquidity and better needle passing performance are achieved, and irritation is reduced during injection.