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发明授权
CN106434704B 参与丹参酮类化合物生物合成的细胞色素P450基因CYP76AH12及其编码产物与应用 失效 - 权利终止

参与丹参酮类化合物生物合成的细胞色素P450基因CYP76AH12及其编码产物与应用
摘要：
本发明涉及一个参与丹参酮生物合成的CYP450基因CYP76AH12及其编码产物与应用，属于药用植物基因工程领域。该基因从丹参中克隆得到，其编码蛋白可以在铁锈醇及其衍生物的7位加一个羰基，是丹参酮生物合成途径中的关键酶。CYP76AH12基因具有SEQ ID No.1所示的核苷酸序列，其编码蛋白具有SEQ ID No.2所示的氨基酸序列。本发明提供的CYP76AH12基因可应用于通过合成生物学或代谢工程技术异源合成和生产二萜类化合物，也可用于调节植物二萜类化合物的生物合成，及通过生物技术提高丹参中丹参酮类化合物的含量。同时该基因也可以用于丹参的杂交育种和优良品种选育。
摘要（英）：

The invention relates to a CYP450 gene CYP76AH12 involved in tanshinone biosynthesis and a coding product and application thereof, belonging to the field of medicinal plant genetic engineering. The gene is cloned from radix salviae miltiorrhizae; in the encoded protein of the gene, a carbonyl can be added to the position 7 of ferruginol or derivative thereof; and the encoded protein is a key enzyme in the path of tanshinone biosynthesis. The CYP76AH12 gene has a nucleotide sequence shown by SEQ ID No.1, and the encoded protein of the gene has an amino acid sequence shown by SEQ ID No.2. The CYP76AH12 gene provided by the invention can be used for heterologously synthesizing and producing diterpene compounds through synthetic biology or metabolic engineering technology and also can be used for adjusting the biosynthesis of plant diterpene compounds and increasing the content of tanshinone compounds through biotechnology. Meanwhile, the gene also can be applied to the crossbreeding and good variety selection of radix salviae miltiorrhizae.

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