The invention provides a pharmaceutical composition and application thereof; the pharmaceutical composition comprises mesenchymal stem cells and a soft tissue filler; the soft tissue filler is prepared from at least one of collagen, hyaluronic acid, calcium hydroxyapatite, polylactic acid, fat and platelet-rich plasma, wherein the mesenchymal stem cells are those sorted according to a cellular surface marker. The pharmaceutical composition is used for improving cellular activity, such as the activity of fibroblasts; compared with the prior art, the pharmaceutical composition has the advantages of low immunogenicity, high acting speed, naturalness, lasting effect, low risk, and good therapeutic effect. The pharmaceutical composition is effective for removing wrinkles, defying age, repairing scars and wounds, and the like.