The invention provides a polyamide-amine/rare earth fluorescent membrane imprint preparation method and application thereof, the preparation method comprises the steps of preparing europium complex; preparing membrane casting solution; preparing polyamide-amine/rare earth fluorescent membrane imprint; removing template molecule in the polyamide-amine/rare earth fluorescent membrane imprint. In the preparation method, the imprinting process reacts on the surface and insides of the membrane material, and avoids the problem of insufficient obstructing force caused by the traditional template molecule only existing on the surface. Adding chitosan, makes the molecule imprinted polymer be embedded in the membrance, and avoids the falling off of the polymer in the membrane cleaning process. The prepared polyamide-amine/rare earth fluorescent membrane imprint has good heat stability, and superior bisphenol A molecule identification performing ability. Combining the molecule membrane imprint technique with a fluorescent detection technique, establishes a new method of simple, fast, and agile operation and high flux BPA content measurement in food products.