The invention relates to a method for using a phase transfer catalyst to conduct deacidification on high acid crude oil. The method comprises the steps of providing the high acid crude oil, wherein an acid value of the high acid crude oil is bigger than 1mg KOH/g; providing the phase transfer catalyst, wherein the phase transfer catalyst is a quaternary ammonium salt (please check the formula defined in the specifications), R1 is a linear chain alkyl group of which a carbon number is 12-16, R 2 and R 3 are short chain alkyl groups of which the carbon number is 1-4, R 4 is a short chain alkyl group or a short chain alkyl phenyl and the carbon number in R 4 is 1-4, and X is chloridion; providing alkaline liquor, wherein the alkaline liquor is chosen from at least one of NaOH solution and Na2CO3 solution, wherein mass percentage of both the NaOH solution and Na2CO3 solution is 1%-4%; mixing the high acid crude oil, the phase transfer catalyst and the alkaline liquor to form a reaction system and using the phase transfer catalyst to transfer petroleum acid in the high acid crude oil to an aqueous phase so that the petroleum acid can conduct a neutralization reaction with the alkaline liquor, wherein the concentration of the phase transfer catalyst in the reaction system is 400-600 mg/L. According to the method for using the phase transfer catalyst to conduct deacidification on the high acid crude oil, the petroleum acid in the high acid crude oil is transferred to the aqueous phase through the phase transfer catalyst so that a purpose of efficient deacidification can be achieved.