The invention discloses a group of detection kits for rapidly detecting an HLA-B27 gene and establishes a relatively efficient and sensitive method for rapidly detecting the HLA-B27 gene. The detection kits for rapidly detecting the HLA-B27 gene are convenient to use, simple to operate and good in detection effect, greatly simplifies the operation process and reduce pollution during operation, have the characteristics of high sensitivity, high specificity and high selectivity and realize typing detection of the HLA-B27 gene. The provided detection method adopts completely closed tube operation and is simple, convenient and quick to operate; detection results are obtained by directly detecting a fluorescence signal Ct value in a PCR direct detection process, post-processing such PCR electrophoresis detection is not needed, the defects that pollution is easily caused and the false positive rate is high with the conventional PCR technology are overcome, and the method can effectively avoid non-specific amplification and is suitable for large-volume sample detection.