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发明授权
CN106521001B 秦川牛microRNA-320a-1基因单核苷酸多态性的检测方法及其应用 失效 - 权利终止

秦川牛microRNA-320a-1基因单核苷酸多态性的检测方法及其应用
摘要：
本发明公开了一种秦川牛microRNA‑320a‑1基因单核苷酸多态性的检测方法及其应用，以待测秦川牛全基因组DNA为模板，以引物对P2为引物，PCR扩增秦川牛microRNA‑320a‑1基因片段；用限制性内切酶PvuII消化PCR扩增产物之后，再对酶切后的扩增片段进行琼脂糖凝胶电泳；根据琼脂糖凝胶电泳结果鉴定秦川牛microRNA‑320a‑1基因rs518926539:C>T的单核苷酸多态性。本发明提供的检测方法为利用microRNA‑320a‑1基因SNP与生长性状的关系进行秦川牛肉用生长性状的标记辅助选择奠定了基础，有利于快速建立遗传资源优良的秦川牛种群。
摘要（英）：

The invention discloses a detection method of single nucleotide polymorphism of a Qinchuan cattle microRNA-320a-1 gene and an application thereof. The detection method comprises the following steps: with the to-be-detected Qinchuan cattle whole-genome DNA as a template and a primer pair P2 as primers, performing PCR amplification of a Qinchuan cattle microRNA-320a-1 gene segment; digesting the PCR amplification product with restriction endonuclease PuvII, and performing agarose gel electrophoresis of the amplified segment after the enzyme digestion; and identifying the single nucleotide polymorphism of Qinchuan cattle microRNA-320a-1 gene rs518926539:C>T according to the result of agarose gel electrophoresis. The detection method disclosed by the invention lays a foundation for the marker assisted selection of the growth character for Qinchuan cattle meat based on the relationship between the microRNA-320a-1 gene SNP and the growth character, and is favorable for quickly establishing a Qinchuan cattle population with good genetic resources.

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