The invention discloses a chiral compound with a selenourea group structure, and also provides a preparation method and application of the selenourea chiral compound. The structure of the new compound is shown as the specification. The compound is used as a chiral shift reagent to detect the optical purity of chiral carboxylic acid, chiral alcohol, chiral amino alcohol and other chiral compounds, has the characteristics of simple operation and large chemical shift difference, thus being a rapid, efficient, convenient and practical detection means.