The invention discloses a cold storage and fresh keeping method of duck eggs. The cold storage and fresh keeping method comprises the following steps of selecting the duck eggs, disinfecting the selected duck eggs, cleaning the disinfected duck eggs, drying the cleaned duck eggs and performing cold storage on the dried duck eggs, wherein the step of disinfecting the selected duck eggs comprises two disinfecting processes in which the duck eggs are respectively treated with ozone water and a disinfectant. According to the cold storage and fresh keeping method disclosed by the invention, disinfecting treatment is performed through the ozone water and the disinfectant, so that bacteria on the surfaces of the shells of the duck eggs are completely killed, the fresh keeping of the duck eggs is facilitated, the storing period of the duck eggs is prolonged, and besides, the influence of the cold storage and fresh keeping method on the quality changes of the duck eggs is small.