The invention relates to a laser sealing method of glass and a kovar alloy. The method comprises the following steps: S1, setting a processing track according to dimension and shape of glass and kovar alloy needed to be sealed, and preparing a welding clamp; S2, performing a pre-stage sealing purifying pre-treatment operation on the glass and kovar alloy and providing Ag-Cu-Ti power or an Ag-Cu-Ti foil as welding flux; S3, uniformly spraying the welding flux in the S2 to the peripheral edges of the kovar alloy to be sealed, and placing the to-be-sealed glass on the kovar alloy; S4, clamping the to-be-sealed body by the welding clamp as required; S5, setting laser processing parameters, wherein a laser performs laser irradiation on the welding flux at the boundary of the glass and the kovar alloy according to the set processing track to prepare a glass-kovar alloy sealing body; and S6, disassembling the welding clamp. The method provided by the invention fully solves the problem that the connecting strength between the glass the kovar alloy is not high, and meanwhile, the sealing body which is high in connecting strength is prepared.