The invention discloses a hirudinaria manillensis lesson seedling disinfection and anti-stress processing method, which comprises the following steps: filling hirudinaria manillensis lesson seedlings into a mesh bag and tightening with a rope; horizontally placing the mesh bag on the dry ground of a farm for 10-15min; immersing the mesh bag together with hirudinaria manillensis lesson into a disinfection solution and continuously soaking for 15-20min; placing the mesh bag with soaked and disinfected hirudinaria manillensis lesson in the middle of or around a cultivating pool of the farm, then untightening the mesh bag of the hirudinaria manillensis lesson seedlings so as to lead the hirudinaria manillensis lesson seedlings to self crawl into the cultivating pool; and after the hirudinaria manillensis lesson seedlings enter the cultivating pool, immediately splashing an anti-stress solution evenly into the whole cultivating pool. By utilizing the method, the safe, effective, pollution-free, green and environment-friendly traditional Chinese herbal disinfection and anti-stress processing can be conducted to the hirudinaria manillensis lesson seedlings, the disease of hirudinaria manillensis lesson seedlings can be effectively prevented, the death rate of the hirudinaria manillensis lesson seedlings can be reduced, so that the survival rate of the seedlings can be improved, and the cultivation benefits can be greatly increased.