The invention provides an aquaculture feed as well as a preparation method and application thereof. The aquaculture feed is prepared from the following components: Peru fish meal, Mauritius fish meal, pet-grade American chicken powder, high gluten flour, bean pulp, cassava starch, fish oil, soybean oil, monocalcium phosphate, a sea fish composite premix, zeolite powder, feed-grade calcium propionate and an antioxidant, wherein the mass percentage of the Mauritius fish meal is 22 to 27 percent. According to the formula, components with relatively low cost replace part of the Peru fish meal, so that the growth performance of micropterus salmoides can be improved, the feed coefficient can be reduced, the feed cost can be reduced, and the survival rate of the micropterus salmoides can be increased. An experimental result shows that the feed coefficient of the aquaculture feed provided by the invention is 0.97; when 50 percent of the Peru fish meal is replaced, the survival rate of the micropterus salmoides is 91.67 percent.