The invention provides a rapid test paper for pathogenic Vibrio parahaemolyticus. A cellulose nitrate membrane is provided with four test lines, i.e., T1, T2, T3 and T4, and a control line C which are successively arranged from the side close to a colloidal-gold pad, wherein the four test lines T1, T2, T3 and T4 are the outer membrane protein, flagellin, an extracellular product and whole bacterium disruption protein of Vibrio parahaemolyticus, respectively, and the control line C close to a water absorbing pad is goat anti-rabbit IgG. According to the invention, a plurality of antigen proteins of pathogenic Vibrio parahaemolyticus are extracted and specifically arrayed, and simultaneously react with a to-be-tested sample on the test paper, and in particular, the test line T4 is provided with the whole bacterium disruption protein of Vibrio parahaemolyticus; thus, the problem that immunological cross-reactions due to common antigens of other bacteria interfere in result determination is overcome, and positive results and cross reactions can be accurately distinguished.