The invention discloses a platelet-rich plasma composition comprising the following components: platelet-rich plasma, botulinum toxin, and a natural tyrosinase inhibitor. The invention also discloses a preparation method and use method of the platelet-rich plasma composition. The platelet-rich plasma composition can retard side effects of the botulinum toxin after use, shortens the recovery period, and prolongs the effect duration time; at the same time, the absorption efficiency of the natural tyrosinase inhibitor such as oxyresveratrol is improved, and the whitening effect can be greatly improved. The beautifying composition combines three beautifying advantages of PRP, the botulinum toxin and the natural tyrosinase inhibitor such as oxyresveratrol, and the combined effect of the three components can produce quick-acting, intermediate-acting and long-term whitening and wrinkle-removing effects.