The invention relates to the field of medicine and discloses inducing liquid and a mehod for inducing human amniotic mesenchymal stem cells to differentiate to cardiomyocyte-like cells. The inducing liquid provided by the invention comprises fetal bovine serum, 5-azacytidine, angiotensin II and a DMEM/F12 culture solution of a transcription growth factor beta1. Two proper components, namely the angiotensin II and the transcription growth factor beta1 are added additionally on the basis of the traditional inductive agent, namely the 5-azacytidine, and the human amniotic mesenchymal stem cells are induced jointly by the combined action of the angiotensin II, the transcription growth factor beta1 and the 5-azacytidine to differentiate to the cardiomyocyte-like cells, so the induced differentiation rate of the human amniotic mesenchymal stem cells can be remarkably increased.