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发明公开
CN106706895A 一种抗FITC荧光单克隆抗体及其免疫层析试纸 无效 - 驳回

一种抗FITC荧光单克隆抗体及其免疫层析试纸
摘要：
本发明涉及一种抗FITC荧光单克隆抗体及其免疫层析试纸，以半抗原FITC与BSA的偶联物FITC‑BSA作为人工完全抗原免疫BALB/c小鼠，并筛选出能稳定分泌抗FITC单克隆抗体的杂交瘤细胞株，进而采用体内诱生腹水的方法制备出具有高亲和力和高特异性的抗FITC荧光的单克隆抗体，上述抗FITC单克隆抗体可用于免疫层析检测，能够准确、敏感、快速并且特异性地检测出由FITC荧光探针标记的蛋白质或核酸分子，从而实现对靶标蛋白或核酸分子的定性和半定量的检测。
摘要（英）：

The invention relates to an anti FITC fluorescence monoclonal antibody and an immunochromatographic test paper; a hapten FITC and BSA conjugate FITC-BSA is used as an artificial complete antigen for immunizing BALB/c mice, and a hybridoma cell strain which can stably secrete the anti FITC monoclonal antibody is screened out, and the anti FITC fluorescence monoclonal antibody having high affinity and high specificity is prepared by an ascites in-vivo induction method; the anti FITC monoclonal antibody can be used for immunochromatographic assay, and can accurately, sensitively, rapidly and specifically detect FITC fluorescent probe-labeled proteins or nucleic acid molecules, so as to realize qualitative and semi-quantitative detection of the target proteins or nucleic acid molecules.

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