The invention discloses a culture medium for aseptically seeding and quickly growing seedling of floribunda paphiopedilum. A seeding culture medium comprises 2g/L hyponex 1#, 2g/L tryptone, 20g/L saccharose and 5.8g/L agar; the pH of the seeding culture medium is equal to 6; a subculture culture medium comprises 2g/L hyponex 1#, 10g/L banana, 100g/L mashed potato, 20g/L saccharose, 1g/L activated carbon and 5.8g/L agar; the pH of the subculture culture medium is equal to 6; a seedling strengthening culture medium comprises 2g/L hyponex 1#, 2g/L tryptone, 0.1mg/L NAA, 0.2mg/L 6-BA, 10g/L banana, 100g/L mashed potato, 20g/L apple juice, 20g/L saccharose, 2g/L activated carbon and 5.8g/L agar; the pH of the seedling strengthening culture medium is equal to 6. The floribunda paphiopedilum includes polyantha and cochlopetalum protospecies and coeno-species thereof. The culture is performed on seedlings in the mature capsule period, preferably, in the period of uncracking capsule and yellowing capsule carpopodium part. Through the aseptic seeding, the germination rate of the seed is increased; a large number of paphiopedilum protospecies and coeno-species in high ornamental value can be produced; the market requirement can be met; the lowering of commodity price of the paphiopedilum is benefited, so that the protection and sustainable utilization for the paphiopedilum protospecies can be enhanced.