The invention belongs to the technical field of drugs and chemicals and discloses a derivative of a natural product selaginpulvilin. The invention further provides a preparation method of the derivative and application of the derivative for preparing anti-inflammatory drugs. The structure of the derivative of the natural product selaginpulvilin disclosed by the invention is shown as a formula (I) or a formula (II), wherein R1 is alkyl, aldehyde, carboxyl, alcohol group, hydroxyl, acyl or hydrogen; R2 is hydrogen or hydroxyl; R3 is alkoxy, acyl or hydroxyl; R4 is halogen, aryl ethynyl, phenyl or substituted phenyl and aryl vinyl; R5 is hydrogen, hydroxyl, phenyl or substituted-phenyl;R6 is hydrogen, phenyl or substituted phenyl;the substituent group is selected from alkoxy, acyl or hydroxyl. The derivative of the natural product selaginpulvilin has an obvious effect of inhibiting phosphodiesterase IV, and has wide application space in preparation of the anti-inflammatory drugs. The structural formula is as shown in the specification.