The invention discloses a polyaryl-substituted imidazole fluorescence probe. The structural formula of the polyaryl-substituted imidazole fluorescence probe is shown in the formula (I). The invention further discloses a preparation method of the polyaryl-substituted imidazole fluorescence probe and application of the polyaryl-substituted imidazole fluorescence probe in the specific detection of a G-quadruplex. The fluorescence probe is simple to prepare, stable in structure and can be applied to the specific detection of a wild c-MYC G-quadruplex nucleic acid secondary structure, the raw materials are easily available, the wild c-MYC G-quadruplex nucleic acid secondary structure in a solution can be rapidly detected by virtue of a fluorescence spectrometer, and the fluorescence probe has no fluorescent responsiveness to mutational c-MYC G-quadruplexes or other DNA secondary structures. The method for the specific detection of the wild c-MYC G-quadruplex nucleic acid secondary structure has the beneficial effects that the operation is simple and convenient, the sensitivity and the specificity are strong, and disadvantages of other detection methods that the cost is high, the equipment requirement is high, the technical operation is relatively complex, and the like can be overcome.