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发明公开
CN106749615A 一种大菱鲆生长催乳素SL基因、重组蛋白及其制备方法 无效 - 驳回

一种大菱鲆生长催乳素SL基因、重组蛋白及其制备方法
摘要：
本发明公开了一种大菱鲆生长催乳素SL基因、重组蛋白及其制备方法。本发明克隆得到了生长催乳素的编码基因，其核苷酸序列为SEQ ID NO:2，催乳素SL多肽的氨基酸序列为SEQ ID NO:1。将生长泌乳素SL基因与PET‑24a表达载体相连接构建表达载体pET‑24a‑SL，转化入表达菌株进行原核表达，后经纯化复性获得大菱鲆生长催乳素重组蛋白，本发明将为研究大菱鲆的生长发育调控机理和开发可应用于生产的调控大菱鲆生长发育的新产品打下良好基础。
摘要（英）：

The invention discloses growth prolactin SL genes for scophthalmus maximus, a recombinant protein and a preparation method thereof. The growth prolactin SL genes, the recombinant protein and the preparation method are characterized in that encoding genes of growth prolactin are obtained by cloning, the nucleotide sequence is SEQ ID NO:2, and the amino acid sequence of prolactin SL polypeptide is SEQ ID NO:1. Growth prolactin SL genes are connected with PET-24a expression vectors to construct expression vectors pET-24a-SL, the expression vectors pET-24a-SL are transformed into expression strains to carry out prokaryotic expression, and then are purified and renatured to obtain the recombinant protein of the growth prolactin of the scophthalmus maximus, and a good basis is laid for studying the growth and development regulation mechanism of the scophthalmus maximus and developing a new product which can be applied in production and regulation of the growth and the development of the scophthalmus maximus.

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