The invention relates to an urban road intersection signal control multi-objective optimization method. The method includes the following steps of: step 1, vehicle exhaust emission modeling: economic losses caused by vehicle exhaust emission is the sum of the economic losses of various pollutants; step 2, vehicle fuel consumption modeling: fuel consumption is converted into economic losses according to the price of gasoline; step 3, vehicle delay modeling; step 4, multi-objective optimization function establishment: three loss situations, namely, vehicle exhaust emission, vehicle fuel consumption and vehicle delay are combined, so that a multi-objective optimization function can be established; and step 5, optimal solution acquisition: a multi-search strategy combined immune multi-objective optimization algorithm is adopted to perform training, so that an optimal solution can be obtained; and step 6, optimal signal timing scheme acquisition: an optimal signal timing scheme is outputted according to the optimal solution. The urban road intersection signal control multi-objective optimization method of the invention has advantages of high adaptability, high feasibility, high practicality, high usability and high practical value.