The invention discloses a folic acid targeted magnetic functionalized tungsten sulfide medicine carrier and a preparation method thereof. The preparation method comprises the following steps: firstly, by using a chemical co-precipitation method, modifying surfaces of tungsten sulfide nano-sheets with MFe2O4 (M is Fe, Co and Ni) magnetic nano-particles, further performing amino-functionalized modification on the magnetic tungsten sulfide nano-sheets, performing targeting modification by using folic acid as a targeting biological molecule through reaction that amido bonds are formed from carboxyl and amino, and finally loading an anti-tumor medicine, thereby obtaining the folic acid targeted magnetic functionalized tungsten sulfide medicine carrier. The folic acid targeted magnetic functionalized tungsten sulfide medicine carrier has the advantages of being controllable in operation process, gentle in reaction condition and easy in large-scale production; by adopting the preparation method, the medicine carrying capacity, blood half life and average retention time of the anti-tumor medicine are remarkably increased; and meanwhile, a medicine transmission system based on the nano composite material can reach and be enriched at focal parts in a targeted manner, and the medicine can be slowly released and is intelligently controllable, thereby achieving the purposes that the toxic or side effects of the anti-tumor medicines are alleviated, the medicine concentration at diseased regions is increased and the treatment effect on tumors is improved.