The invention relates to the technical field of tissue culture and discloses a method for acquiring pepper somatic embryogenesis by taking testa as explant. The method provided by the invention comprises the following steps: sterilizing mature pepper seeds; culturing into pepper seedlings; separating testa from seedling cotyledons; and performing callus induction culture and differentiation culture on the testa in turn, thereby acquiring the pepper somatic embryogenesis. According to the method provided by the invention, the testa is selected as the explant, the formula of the callus induction medium and differential medium is adjusted, and MS is taken as a basal culture medium and the ingredients, such as, activated carbon, polyvinylpyrrolidone and 2,4-D are added, so that the differentiation rate of the pepper somatic embryogenesis is as high as 20% and the method has significance in researching high throughput pepper seedling propagation and establishing transgenosis breeding system.