The invention discloses a preparation method of a holographic 3D (three-dimensional) display screen of a cadmium selenide quantum dot-doped liquid crystal material. The preparation method comprises the following steps of according to a certain ratio, doping the cadmium selenide quantum dots into liquid crystal to form a holographic material; injecting the material into two pieces of ITO (indium tin oxide) glass, so as to form the holographic 3D display screen. The cadmium selenide quantum dots are doped into the liquid crystal to form the holographic material, the object light and reference light of a system are provided by laser, and the readout light is provided by visible light. The prepared holographic display material has the advantages that a carrier is provided for the holographic 3D display, the dynamic refreshing characteristic is realized in the holographic display system, the diffraction efficiency is improved, and the response time is shortened; the holographic 3D display screen can be applied to the fields of printing of holographic maps, holographic optic discs, real-time dynamic holographic display, holographic photographing and the like.