利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN106834262B 一种亚油酸异构酶突变体及其应用 有权

一种亚油酸异构酶突变体及其应用
摘要：
本发明公开了一种亚油酸异构酶突变体及其应用，所述突变体由氨基酸序列如SEQ ID NO：1所示的亚油酸异构酶第62位蛋氨酸突变为丙氨酸或甘氨酸，和/或第295位丝氨酸突变为丙氨酸、亮氨酸或缬氨酸得到。本发明亚油酸异构酶突变体与野生型酶相比，催化亚油酸获得反‑10,顺‑12共轭亚油酸的酶活力得到了大大的提高，特别是双突变时，如M62A/S295L，M62A/S295A，M62A/S295V，M62G/S295L，M62G/S295A，M62G/S295V，能够提高10倍左右，具备良好的工业应用前景。
摘要（英）：

The invention discloses a linoleate isomerase mutant and application thereof, and the linoleate isomerase mutant is obtained by mutation of 62th-site methionine of linoleate isomerase with an amino acid sequence as shown in SEQIDNO:1 into alanine or glycine, and mutation of 295th-site serine into alanine, leucine or valine. The enzyme activity for catalysis of linoleic acid for preparation of trans-10,cis-12 conjugated linoleic acid of the linoleate isomerase mutant can be greatly improved compared with that of a wild-type enzyme, especially during double mutation such as M62A / S295L, M62A / S295A, M62A / S295V, M62G / S295L, M62G / S295A and M62G / S295V, the enzyme activity can be increased by about 10 times, and the linoleate isomerase mutant has good prospects for industrial application.

公开/授权文献
0/0