The invention provides a preparation method of anthracene-doped polyphenyl acetylene-graphene OLED photoelectric particles. The method comprises grinding anthracene groups and polyphenyl acetylene under frozen conditions, during the grinding process, introducing graphene conductive agent, taking graphene as an external skeleton to form a non-coplanar scroll structure to reduce fluorescence quenching. Therefore, the high fluorescence quantum efficiency can be kept and meanwhile, the performance of the polyphenyl acetylene can be effectively regulated. The preparation method of the an anthracene-doped polyphenyl acetylene-graphene OLED photoelectric particles overcomes the intrinsic defect of crystallization proneness of small molecules such as anthracene serving as light emitting units in the prior art, increases the service life and the thermal stability of devices and is simple in synthetic processes of composite materials, low in cost of raw materials and capable of meeting the requirements of industrialized development.