The invention provides an online energy regulation method of a wind, light and hydrogen comprehensive energy source system. The online energy regulation method is characterized by comprising an off/on grid operation condition of the wind, light and hydrogen comprehensive energy source system, an operation condition of a fuel cell-hydrogen production-hydrogen storage self closed-loop system, operation of an off/on grid wind and light-hydrogen production-hydrogen storage open loop system, operation of an off/on grid wind and light-hydrogen production-hydrogen storage-fuel cell closed loop system and the like. By adopting the method provided by the invention, online energy regulation of the wind, light and hydrogen comprehensive energy source system is analyzed and the effectiveness of the method on the online energy regulation of the wind, light and hydrogen comprehensive energy source system is sufficiently reflected, so that wind power and photovoltaic absorptive capabilities of a power grid are improved; the online energy regulation method has the advantages of good stability, high adaptability, high actual application value and the like.