The invention provides a preparation method of health-care type lactic acid bacteria fermented yoghurt, belongs to the technical field of food processing and in particular relates to the preparation method of the health-care type lactic acid bacteria fermented yoghurt. The preparation method comprises the following steps: adding water into soybean sprouts; pulping the soybean sprouts into soybean sprout pulp by a pulping machine; uniformly mixing the soybean sprout pulp with fresh milk; keeping heat and carrying out enzymolysis at 45 DEG C to 50 DEG C for 0.5h to 1h; then homogenizing; after homogenizing, sterilizing and cooling, inoculating a yoghurt fermentation agent and fermenting for 5h to 8h; cooling, crushing curd, filling and carrying out after-ripening to obtain a finished product. According to the preparation method provided by the invention, the soybean sprouts are added in a production process of the yoghurt; compared with a process of fermenting the yoghurt with a traditional method, a soybean sprout enzymolysis step is increased, so that the yoghurt produced by the method has original nutrients and functional components of traditional yoghurt, and various functional components including gamma-aminobutyric acid, flavonoid aglycone and the like are also increased, so that the health-care effect is more prominent.