The invention discloses an individualized 3D (three-dimensional) printed bone tissue engineering stent with a mosaic structure. The stent is characterized in that a shape and a structure of a large area of bone defect of a jaw are rebuilt through 3D modeling. The stent comprises two parts, wherein one part is an outer outline stent used for restoring the shape and the structure of a cranio-facial bone defect, and the other part is an inner tubular stent used for promoting formation of blood vessels. The bone tissue engineering stent with the maintained shape and structure on the outer part and promoted formation of blood vessels in the inner part is formed by inoculating factors for promoting ossification, such as mesenchymal stem cells (MSCs) and/or a loaded bone morphogenetic protein (BMP), onto the outer stent, and inoculating factors for promoting the formation of the blood vessels, such as endothelial cells (ECs) and/or a loaded vascular endothelial cell growth factor (VEGF), onto the inner stent, so that stem cell proliferation, ossification differentiation, and angiopoiesis of a tissue engineering compound are facilitated, and quality and speed of osteanagenesis are improved.