利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN106866796A 草鱼呼肠孤病毒Ⅱ型S9基因编码的重组蛋白及其应用 无效 - 驳回

草鱼呼肠孤病毒Ⅱ型S9基因编码的重组蛋白及其应用
摘要：
本发明公开了一种草鱼呼肠孤病毒Ⅱ型S9基因编码的重组蛋白及其应用。所述重组蛋白的氨基酸序列如SEQ ID NO:2所示，编码该蛋白的核苷酸序列如SEQ ID NO:1所示。本发明获得的重组蛋白具有较好的免疫原性，与GCRV其它结构蛋白相比，其诱导免疫动物产生的特异性抗体效价更高。进一步试验表明，用VP6蛋白免疫草鱼，可使草鱼诱导产生较高的特异性抗体，并能对草鱼呼肠孤病毒强毒株的攻击起到一定的免疫保护作用。因此，对GCRV Ⅱ型VP6蛋白的研究和应用，对开发草鱼出血病新型疫苗及免疫学检测试剂盒具有极其重要的意义，为草鱼出血病提供新的有效解决途径。
摘要（英）：

The invention discloses a recombinant protein coded by GCRV (grass carp reovirus) II type S9 genes and application thereof. The amino acid sequence of the recombinant protein is shown as SEQ ID NO:2. The nucleotide sequence for coding the protein is shown as SEQ ID NO:1. The obtained recombinant protein has better immunogenicity. Compared with CGRV proteins of other structures, the recombinant protein has the advantage that the valence of specific antibodies generated by immune animals induced by the protein is higher. Further experiments show that when VP6 proteins are used for immunizing grass carps, the grass carps can be induced to generate higher specific antibody; a certain immune protection effect is achieved on the GCRV strong virus plant attack. Therefore the development and the application of the GCRV II type VP6 protein have very important significance on developing novel grass carp hemorragic disease vaccine and immunological detection kits, and a novel effective solution path is provided for grass carp hemorragic diseases.

0/0