The invention provides fusarium disease pathogenic bacteria RT-PCR (reverse transcription-polymerase chain reaction) detection primer and probe combination and a kit. A nucleotide sequence of the primer and probe combination is shown as SEQ ID NO:1-12. The kit disclosed by the invention can quickly, specifically, flexibly and stably perform molecular diagnosis on cornea fusarium disease pathogenic bacteria. Based on the establishment of a reliable fusarium disease pathogenic bacteria detecting system, a competitiveness internal reference is pertinently designed to guarantee that negative results are genuine and believable. Meanwhile, the kit provided by the invention includes an improved DNA extracting system, can quickly and effectively finish a DNA extracting process and enables detection to be quicker and more convenient.