The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving the yield of bacitracin produced through microbial fermentation. According to the method, by optimizing an organic composite nitrogen source and fermentation conditions, batch fermentation unit stability is ensured, and the content of impurities F is lowered; superparamagnetism microspheres are added in the bacitracin synthesis period to adsorb bacitracin in a fermentation solution, and therefore feedback restraint of a fermentation production for producing bacteria is lowered. After fermentation is finished, the fermentation unit of bacitracin can reach 1228 U/ml, the yield of bacitracin can be improved by 123% compared with the yield obtained before optimization, the yield is improved, energy consumption is lowered, and waste discharge is reduced.