The invention discloses a bonded-phase chromatography-tandem mass spectrometry for a chiral analysis on nicotine in a smoking cessation drug. The bonded-phase chromatography-tandem mass spectrometry is characterized by comprising the following steps: drying, smashing and sieving smoking cessation drug samples; after the samples are soaked by sodium hydroxide solution, extracting the solution with methyl tertiary butyl ether and a methanol solution; after matrix solid-phase dispersion extraction and purification are performed on extract liquor, making an analysis with two chiral columns by a tandem bonded-phase chromatography-tandem mass spectrometry; quantitatively detecting a proportion of S-(-)-nicotine and R-(+)-nicotine in the smoking cessation drug by adopting a peak area normalization method. According to the detection method provided by the invention, the extraction and purification methods of the samples are simple and efficient, so that the pollution of a sample solution to a chromatographic column can be reduced; the detection method of the bonded-phase chromatography-tandem mass spectrometry has the advantages that the sensitivity is high, the specificity is good, false positive can be eliminated, the analysis time is short, the requirement for the chiral analysis on the nicotine in a large batch of smoking cessation drug samples can be met.