The invention discloses an optical scattering simulation model construction method and an application thereof. A scattering medium of a to-be-constructed model is disposed under a microscope to carry out three-dimensional stereo scanning imaging to acquire a three-dimensional structure image of the scattering medium; after each two-dimensional structure image is converted into a gray image matrix, and amplitude normalization processing is carried out to acquire normalization image matrixes; a phase difference weight is set, the phase difference weight is multiplied to each normalization image matrix to acquire optical phase difference distribution matrixes, and the optical scattering simulation model is formed by all the optical phase difference distribution matrixes. The method is advantaged in that corresponding value simulation models can be simply and conveniently acquired while experiment samples are made, the process is closer to mutual effects of light beams and biological tissues in experiments, and experiment efficiency can be better simulated and improved.