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发明公开
CN106906148A 一种球孢白僵菌的培养基、球孢白僵菌的培养方法 无效 - 驳回

一种球孢白僵菌的培养基、球孢白僵菌的培养方法
摘要：
本发明公开一种球孢白僵菌的培养基、球孢白僵菌的培养方法，所述培养基包括液体培养基和固体培养基，所述液体培养基的组分按重量份数计包括葡萄糖5‑15份、酵母粉2‑8份、马铃薯汁3‑10份、蛋白胨5‑20份、蚕粉5‑10份、硫酸二氢钾1‑5份、头孢霉素1‑5份、无菌水20‑80份；所述固体培养基的组分按重量份数计包括葡萄糖5‑15份、玉米粉5‑15份、蚕粉5‑10份、乳糖10‑20份、麦麸10‑20份、头孢霉素1‑5份。通过本申请所述的培养基得到的球孢白僵菌分生孢子具有较高的侵染寄主昆虫的毒力，杂菌污染少，成功率高，繁殖周期缩短，球孢白僵菌分生孢子的质量稳定。
摘要（英）：

The invention discloses a medium and culture method of beauveria bassiana. The medium includes a liquid medium and a solid medium. The liquid medium comprises the following components, by weight part, 5-15 parts of glucose, 2-8 parts of yeast powder, 3-10 parts of potato juice, 5-20 parts of peptone, 5-10 parts of silkworm powder, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of cephalosporin and 20-80 parts of sterile water. The solid medium comprises the following components, by weight part, 5-15 parts of glucose, 5-15 parts of corn flour, 5-10 parts of silkworm powder, 10-20 parts of lactose, 10-20 parts of wheat bran and 1-5 parts of cephalosporin. Beauveria bassiana conidia obtained from the medium have great power of invading host insects, are less polluted by other bacteria, and are high in success rate. The breeding period is short, and the quality of beauveria bassiana conidia is stable.

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