The invention discloses a method for promoting Luteolin accumulation in honeysuckle bud by using fungi, and the method the comprises the steps of cultivating a honeysuckle by cutting seedling, and inoculating fungi to the honeysuckle branch in the cutting process to improve Luteolin content in the honeysuckle bud. The fungi are cynodon dactylon and Aspergillus oryzae. The fungi is used to process the honeysuckle plant, making the honeysuckle grow in the accompany of the fungi's growth; the fungi grows in the stem base of the honeysuckle; the growth of the fungi can promote the accumulation of secondary metabolites in the honeysuckle bud, thereby raising honeysuckle flower quality. The raw material source is wide; the operation is convenient; the method can be easily carried out; and the method has an important meaning in raising honeysuckle flower quality.