The invention relates to the technical field of genetic engineering and discloses a recombinant saccharomyces cerevisiae strain as well as a construction method and application thereof. The recombinant saccharomyces cerevisiae realizes production of 8-dimethyl isopentenyl naringenin with tyrosine as a substrate, in the recombinant saccharomyces cerevisiae strain, rhodosporidium toruloides is sourced from a TAL gene, parsley is sourced from the TAL gene, arabidopsis is sourced from a CHS gene, sweet mandarin is sourced from a CHI gene and sophora flavescens is sourced from an N8DT gene combination, thereby having the effect of producing the 8-dimethyl isopentenyl naringenin with high yield, and experiments show that the yield of the 8-dimethyl isopentenyl naringenin can reach 36.7mu g/L. The recombinant saccharomyces cerevisiae strain is utilized for producing the 8-dimethyl isopentenyl naringenin, cost is low, byproducts are fewer and pollution is low compared with plant extraction and chemical synthesis, a feasible method is provided for production of the 8-dimethyl isopentenyl naringenin and related products thereof, and a foundation is laid for biosynthesis of icaritin and icariin.