The invention discloses a strain of auricularia auricula, which is named Li'er No.3 and belongs to the genera of auricularia auricula. The strain is preserved in China General Microbiological Culture Collection Center, wherein the preservation address is Chaoyang District of Beijing, Beichen West Rd., 1st yard, No.3, at Jul 5th, 2016, and the accession number is CGMCC No. 12525. The Li'er No.3 strain has the characters of solitary single-sheet fruiting body, moderate lug size, being free of root, being bowl-like or ear-like in shape, being grey in color of back side and black in abdomen surface when dried, being glossy, having significant color difference between the front and the back side, having round and complete edge of the lugs, and having non-obvious ribs and collaterals on the back side, and the like. The single sheet rate during growth of the auricularia auricular reaches more than 90%. Defects of poor resistance of "Xin Ke" and poor lug shapes in "916" are improved. The strain of auricularia auricula has excellent promotion prospect.