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发明公开
CN106947748A 一种超耐Mn2+的细菌漆酶、重组载体、重组菌、酶制剂、复合酶系及其制备方法与应用 失效 - 权利终止

一种超耐Mn<base:Sup>2+</base:Sup>的细菌漆酶、重组载体、重组菌、酶制剂、复合酶系及其制备方法与应用
摘要：
本发明涉及一种超耐Mn2+的细菌漆酶、重组载体、重组菌、酶制剂、复合酶系及其制备方法与应用，属于生物技术领域。本发明来源于宏基因组的细菌漆酶lac1542，编码该漆酶的基因核苷酸序列如SEQ ID NO.1所示，该细菌漆酶以ABTS为底物时最适pH为4.0；最适反应温度为75℃且高温下稳定性强；100mmol/L的Mn2+仍能对该酶起激活作用；该细菌漆酶和锰过氧化物酶、灰盖鬼伞过氧化物酶组成复合酶系在低浓度下即可降解木质素达71.5％；该细菌漆酶和灰盖鬼伞过氧化物酶复合酶系对甲基橙的脱色率达到54.8％。本发明的细菌漆酶可作为一种新的酶源用于造纸、染织和环保领域，具有广阔的工业应用前景。
摘要（英）：

The invention relates to ultra-Mn2<+>-resistant bacterial laccase, a recombinant vector, recombinant bacteria, an enzymic preparation, a compound enzyme system and a preparation method and an application thereof and belongs to the biotechnical field. The ultra-Mn2<+>-resistant bacterial laccase is originated from bacterial laccase lac1542 of metagenome, and the gene nucleotide sequence of the laccase is as shown in SEQ ID NO.1. The optimum pH of the bacterial laccase taking ABTS as a primer is 4.0; the optimum reaction temperature is 75 DEG C and the bacterial laccase is highly stable at a high temperature; 100mmol/L Mn2<+> still can activate the enzyme. Bacterial laccase, manganese peroxidase and coprinus cinereus peroxidase form the compound enzyme system which can degrade 71.5% of lignin at a low concentration; the decolourization ratio of the bacterial laccase and coprinus cinereus peroxidase compound enzyme system on methyl orange can reach 54.8%. The bacterial laccase provided by the invention can be used in the field of papermaking, dyeing and weaving and environmental protection as a novel enzyme source and has a wide industrial application prospect.

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