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发明公开
CN106947816A 一种斜带石斑鱼亲子鉴定微卫星荧光多重PCR的方法 有权

一种斜带石斑鱼亲子鉴定微卫星荧光多重PCR的方法
摘要：
本发明公开了一种斜带石斑鱼亲子鉴定微卫星荧光多重PCR的方法，包括以下步骤：(1)斜带石斑鱼个体DNA提取；(2)斜带石斑鱼多态性微卫星引物的筛选；(3)斜带石斑鱼8重PCR条件的优化和扩增；(4)亲子鉴定；本发明方法利用微卫星标记与多重荧光PCR技术结合，筛选了8个高度多态性微卫星位点，组建一个8重荧光PCR体系。利用斜带石斑鱼家系对8重荧光PCR体系进行验证，本发明所述鉴定方法操作简单、快捷、费用低廉，可以在斜带石斑鱼种质鉴定，家系管理和良种选育进行推广应用，并为增殖放流效果评估提供了一种新的技术手段。
摘要（英）：

A microsatellite multiple fluorescent PCR method for E. coioides paternity testing is disclosed. The method includes (1) a step of extracting individual DNAs of E. coioides; (2) a step of screening E. coioides polymorphic microsatellite primers; (3) a step of optimizing E. coioides eightfold PCR conditions and performing amplification; and (4) a step of performing paternity testing. The method combines microsatellite marking and a multiple fluorescent PCR technique, eight polymorphic microsatellite loci are screened and an eightfold fluorescent PCR system is built. The eightfold fluorescent PCR system is verified by utilizing the E. coioides family. The method is simple and rapid to operate and low in cost, can be popularized and applied in E. coioides germplasm identification, family management and improved-variety selection, and provides a novel technical means for assessment of enhancement and releasing effects.

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