The invention discloses a preparation method of a silver catalyst used for alkenes epoxidation. The method comprises the following steps: 1) providing an aluminum oxide carrier; 2) immersing the carrier by utilizing a mixed solution of a silver-containing compound and organic amine; 3) carrying out solid-liquid separation on a mixture immersed in step 2) to obtain solid particles; 4) drying the solid particles in step 3), wherein the step of drying is carried out in procedures of at least two phases, freezing and drying are carried out in a first phase and low-temperature drying is carried out in a second phase; and 5) activating the dried solid particles. According to the method disclosed by the invention, the carrier is not immersed through utilizing a solution containing rhenium. Therefore, the prepared silver catalyst has improved activity and selectivity when being used for preparing ethylene oxide through ethylene oxidation. The invention further relates to the catalyst prepared by the preparation method and application of the catalyst to production of the ethylene oxide through ethylene oxidation.