The invention discloses a method for gene gun-mediated genetic transformation of moso bamboos. The method includes the steps: moso bamboo mature embryo acquisition and pre-culture; extraction of plasmid containing target genes; gene gun parameter setting; resistance screening; transformed plant identification; positive plant culture; transplantation. The method further includes a formula of a special culture medium in processes of transformation and induction. The method solves the problem of difficulty in genetic transformation of the moso bamboos, the period of acquiring transgenic bamboos can be effectively shortened (resistant seedlings are acquired after screening is performed for one month, and positive plants can be acquired for two to four months), and the probability of acquiring the transgenic bamboos is increased. The method is good in repeatability, plays an important role in industrial bamboo genetic improvement, bamboo function genomics research and the like, and has a wide application prospect.