The invention relates to an analysis and detection method of palbociclib intermediates and impurities in the palbociclib intermediates, in particular to a mass analysis method of the palbociclib intermediates. The method comprises the step of conducting mass analysis on the palbociclib intermediates by the adoption of an efficient liquid chromatography. According to the method, an adopted chromatographic column is a chromatographic column C18, and the column temperature is 28-45 DEG C. According to the method, by the adoption of the liquid chromatography, the palbociclib intermediates and the impurities in the palbociclib intermediates are effectively separated under certain chromatographic conditions, and the content of the impurities in the palbociclib intermediates can be accurately determined by the method.