The invention relates to pinolenic acid and/or pine-seed oil pine-seed polypeptide micro-capsule powder and a preparation method thereof and belongs to the field of food. The powder is prepared from the following raw materials in parts by weight: 5-15 parts of water-soluble core material, 5-40 parts of mixed oily core material, 2-20 parts of emulsifying agent, 50-90 parts of wall material, 0.5-10 parts of oil-in-water type emulsifying agent and 0.01-1 part of anti-caking agent. The pinolenic acid and/or pine-seed oil pine-seed polypeptide micro-capsule powder has high stability and fluidity, is free from phenomena of floating oil, sediment and bottle sticking, can effectively promote the absorption for pine-seed polypeptide, can protect the pine-seed polypeptide and can exert the specific effects of the two materials including pine-seed polypeptide and pinolenic acid, and meanwhile, the pinolenic acid monoglyceride is used as the emulsifying agent in the invention and the emulsifying agent can achieve an excellent emulsifying effect, can increase the content of the pinolenic acid, has an emulsifying function and also can exert the effect of the pinolenic acid.