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CN106978503A 一种用于检测沙门氏菌的LAMP检测用引物组及检测方法 无效 - 驳回

一种用于检测沙门氏菌的LAMP检测用引物组及检测方法
摘要：
本发明属于生物检测技术领域，涉及一种用于检测沙门氏菌的LAMP检测用引物组，LAMP检测引物组包括四条特异性引物，分别为正向内侧引物FIP:SEQ ID NO.1、反向内侧引物BIP:SEQ ID NO.2、正向外侧引物F3:SEQ ID NO.3和反向内侧引物B3:SEQ ID NO.4。检测方法包括：1、提取待测样品DNA；2、配制LAMP检测反应体系；3、以步骤1中提取的DNA为模版进行LAMP扩增反应，用实时浊度法鉴定扩增结果。本发明对沙门氏菌定性检测灵敏度可达到6.13×102CFU/mL(细菌浓度)，其灵敏度比普通PCR高10倍且反应结果易于观察。本发明设计的LAMP引物组和建立的沙门氏菌的LAMP检测方法具有特异性好、灵敏度高、快速、费用低廉等优点,可应用于基层实时、快速、准确的检测霍乱弧菌。
摘要（英）：

The invention belongs to the technical field of biological detection and relates to an LAM detection primer group for detecting salmonella. The LAM detection primer group comprises four specific primers, which are a positive inner primer FIP:SEQ ID NO.1, a negative inner primer BIP:SEQ ID NO.2, a positive outer primer F3:SEQ ID NO.3 and a negative inner primer B3:SEQ ID NO.4 separately. The detection method comprises the steps of (1) extracting a to-be-detected sample DNA; (2) preparing an LAM detection reaction system; and (3) carrying out LAM amplified reaction by adopting the DNA extracted in the step (1) as a template and identifying the amplification result by using a real-time turbidity method. The qualitative detection sensitivity on the salmonella can reach 6.23*10<2>CFU/mL (bacterial concentration), the sensitivity is 10 times higher than that of ordinary PCR and the reaction result is easy to observe. The LAM primer group and the built detection method for the salmonella have the advantages of being good in specificity, high in sensitivity, fast and low in cost, and can be applied to basic-level real-time, fast and accurate detection of the salmonella.

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