The invention discloses a procalcitonin-C-reactive protein two-in-one detection kit. The kit is provided with a test paper card and is characterized in that the test paper card is orderly provided with a PVC plate, a sample pad, a binding pad, a nitrocellulose film and a water absorbent pad from bottom to top, the binding pad adsorbs two monoclonal antibody-microsphere coupled complexes such as a rare earth Eu<3+> fluorescent microsphere-labeled anti-procalcitonin antibody and a rare earth Eu<3+> fluorescent microsphere-labeled anti-C-reactive protein antibody, the rare earth fluorescent microsphere has the diameter of 150nm, contains rare earth lanthanide Eu<3+>, is stable in a ground state and can emit fluorescence at a wavelength of 615 nm under the excitation light of 337 nm, the monoclonal antibodies are purified mixed monoclonal antibodies and are derived from monoclonal antibody cell strains specifically for antigen epitopes such as 2 to 6 different procalcitonins and C-reactive proteins. The kit has the advantages of operation simpleness, fast reaction rate, high sensitivity and strong specificity.