The invention belongs to the methods in the food beverage processing field, and concretely discloses a preparation method of selenium-rich fruit distilled liquor. The preparation method comprises the steps of producing selenium-rich fruits, picking and selecting, squeezing, carrying out temperature controlled fermentation, separating, clarifying, carrying out lactic acid fermentation, finely filtering, distilling for the first time, distilling for the second time, filtering, ageing and filling. According to the preparation method of the selenium-rich fruit distilled liquor provided by the invention, the selenium element content in the fruits are remarkably improved, then high-quality Baijiu is obtained through fermentation and distillation processes, various nutritional ingredients in the fruits and selenium element beneficial for a human body can be maximally retained in the obtained Baijiu, and the functional ingredient selenium element can be quickly and comprehensively absorbed by the human body. The preparation method is suitable for being applied to producing selenium-rich fruit Baijiu and selenium-rich health care wine.