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发明公开
CN106995798A 一种CIK细胞的诱导培养试剂及其培养方法 无效 - 驳回

一种CIK细胞的诱导培养试剂及其培养方法
摘要：
本发明公开了一种CIK细胞的诱导培养试剂，该试剂包括试剂A：50ng/ml抗CD3单克隆抗体；试剂B：1000IU/ml IFN‑γ；试剂C：1000IU/ml IL‑2和20ng/ml IL‑15；试剂D：100IU/ml IL‑1；试剂E：淋巴细胞分离液40ml。同时公开了利用上述试剂对CIK细胞的诱导培养方法本发明可以实现简单，高效，稳定的诱导CIK细胞；在经典制备方案中加入IL‑15更好的提高了CIK细胞的增殖数量和γ‑IFN的分泌，提高CIK细胞的抗瘤作用；CIK细胞培养过程中细胞诱导质量高以及细胞增殖数量的稳定性好。
摘要（英）：

The invention discloses an inducing culture reagent for CIK (Cytokines Induced Killer) cells. The reagent is prepared from a reagent A: 50ng/ml anti-CD3 monoclonal antibody; a reagent B: 1000IU/ml IFN-gamma; a reagent C: 1000IU/ml IL-2 and 20ng/ml IL-15; a reagent D: 100IU/ml IL-1; a reagent E: 40ml of lymphocyte separation medium. The invention also discloses an inducing culture method for the CIK cells by using the reagent. The reagent and the method, provided by the invention have the advantages that simple, high-efficient and stable induced CIK cells can be realized; by adding IL-15 in a classic preparation solution, multiplication number of the CIK cells and secretion of gamma-IFN can be better improved, and the anti-tumor effect of the CIK cells is improved; the cell inducing quality during CIK cell culture is high and the stability of cell multiplication number is good.

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